›› 2012, Vol. 24 ›› Issue (1): 20-24.doi: 10.3969/j.issn.1004-616x.2012.01.005

• 论著 • Previous Articles     Next Articles

In vitro anti-tumor activity of Forsythia suspensa extract

YAN Xi，ZHAO Lian-mei，SUN Jia-wei，SHAN Bao-en   

  1. 1. Research Center, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011; 2. The Department Of Pulmonology, the First Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China
  • Received:2011-05-09 Revised:2011-10-02 Online:2012-01-30 Published:2012-01-30
  • Contact: SHAN Bao-en

PDF (PC)

1024

Abstract: OBJECTIVE: To observe the effects of the ethanol and water extracts of Forsythia suspensa in which root，stem and leaf were tested， on the proliferation and apoptosis of human esophageal carcinoma cells in vitro， and to investigate its probable mechanisms. METHODS： The ethanol and water extracts of the root ，leaf and fruit of Forsythia suspensa were prepared. The growth-inhibitory effect of extracts of Forsythia suspensa on esophageal cancer cells were examined by MTT assay. Morphological changes of cancer cells were studied by light microscope and Wright-Giemsa staining. The apoptotic rate was analyzed with Annexin/PI double staining. Western blotting was employed to assess the changes of PARP expression. RESULTS ： All extracts of Forsythia suspensa could inhibit the proliferation of esophageal cancer cells，and the inhibitory effect of FSEER was the most obvious among them. After treatment with FSEER，TE-13 cells showed typical apoptosis morphological changes and the apoptotic rate was increased as compared to control group (P<0.05). Meanwhile， the cleavage of PARP was visible， and all of these changs were in a time-and dose-dependent manner. CONCLUSION： FSEER could significantly inhibit human esophageal carcinoma cell growth in vitro by inducing apoptosis.

Key words: Forsythia suspensa, anti-tumor, anti-proliferation, apoptosis